Jun
9
Analyzing RNA-seq data (DEG, Alt. splicing and splicing efficiency analysis)
The steps I take to analyse RNA-seq data in the lab are :
Checking mapping qualities, e.g. running fastQC on fastq files. Check the fastQ files to see if any reads cotain the primer (adapter sequence). See Note 3 ↓. This could also be seen in the fastQC (quality checking) results (see fig 1 & 2).
