gacatag

I am Ali Oghabian, a Post-doc researcher/Bioinformatician working in the Neuromuscular diseases and Genetic Determinants of Osteoporosis research labs, at the University of Helsinki and Folkhälsan Research Center. The main purpose of this blog is to document and share technical Bioinformatics notes based on challenges that I face everyday in the lab.

Analyzing RNA-seq data (DEG, Alt. splicing and splicing efficiency analysis)

The steps I take to analyse RNA-seq data in the lab are :

Checking mapping qualities, e.g. running fastQC on fastq files. Check the fastQ files to see if any reads cotain the primer (adapter sequence). See Note 3 ↓. This could also be seen in the fastQC (quality checking) results (see fig 1 & 2).